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cleaved caspase 3
Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 42355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleaved+caspase+3/Caspase-3+Antibody/pmc13675316-5-0-3
Average 99 stars, based on 42355 article reviews
Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 42355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleaved+caspase+3/Caspase-3+Antibody/pmc13675316-5-0-3
Average 99 stars, based on 42355 article reviews
cleaved caspase 3 - by Bioz Stars,
2026-11
99/100 stars
Images
1) Product Images from "miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis"
Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis
Journal: Molecular Biology Reports
doi: 10.1007/s11033-026-12913-5
Figure Legend Snippet: MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
Techniques Used: In Vitro, In Vivo, Western Blot, Analysis, Control, Transfection, Virus, Negative Control
Figure Legend Snippet: FOXO1 and PTEN mediate the regulatory effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-FOXO1 or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. (B) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-PTEN or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion
Techniques Used: Activation Assay, Western Blot, Analysis, Expressing, Transfection, Negative Control
Figure Legend Snippet: Akt and mTOR are involved in mediating the effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with MK2206 or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( B ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with rapamycin or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion
Techniques Used: Activation Assay, Western Blot, Analysis, Expressing, Transfection, Negative Control
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