Review



cleaved caspase 3  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc cleaved caspase 3
    MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 42355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+caspase+3/Caspase-3+Antibody/pmc13675316-5-0-3
    Average 99 stars, based on 42355 article reviews
    cleaved caspase 3 - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis"

    Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

    Journal: Molecular Biology Reports

    doi: 10.1007/s11033-026-12913-5

    MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
    Figure Legend Snippet: MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion

    Techniques Used: In Vitro, In Vivo, Western Blot, Analysis, Control, Transfection, Virus, Negative Control

    FOXO1 and PTEN mediate the regulatory effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-FOXO1 or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. (B) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-PTEN or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion
    Figure Legend Snippet: FOXO1 and PTEN mediate the regulatory effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-FOXO1 or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. (B) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-PTEN or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion

    Techniques Used: Activation Assay, Western Blot, Analysis, Expressing, Transfection, Negative Control

    Akt and mTOR are involved in mediating the effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with MK2206 or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( B ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with rapamycin or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion
    Figure Legend Snippet: Akt and mTOR are involved in mediating the effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with MK2206 or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( B ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with rapamycin or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion

    Techniques Used: Activation Assay, Western Blot, Analysis, Expressing, Transfection, Negative Control

    Related Articles

    Cell-Signaling:

    Article Title: Mitofusin 2 orchestrates mitochondrial metabolic homeostasis and suppresses cutaneous squamous cell carcinoma by stabilizing ER-mitochondria contacts.
    Article Snippet: Extended author information available on the last page of the article Abstract This study aimed to elucidate the biological role of Mitofusin 2 (Mfn2) in cutaneous squamous cell carcinoma (cSCC) and evaluate how its regulation of mitochondria-associated membranes (MAMs) may suppress tumor progression by reshaping mitochondrial metabolic homeostasis and apoptosis.. We compared Mfn2 expression between normal keratinocytes (HaCaT) and cSCC cells (A431), establishing a stable Mfn2-overexpressing (Mfn2-OE) A431 model.. Structural impacts on ER-mitochondrial contacts and MAMs were evaluated using immunofluorescence, STED super-resolution microscopy, TEM, and subcellular fractionation (VDAC1/IP3R/Grp75).

    Article Title: Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21
    Article Snippet: Caspase‐3 , 9662S , Cell Signaling Technology (CST) , AB_331439. .. Cleaved Caspase‐3 , 9661S , Cell Signaling Technology (CST) , AB_2341188. .. Bak , 12105 , Cell Signaling Technology (CST) , AB_2716685.

    Article Title: Acetaminophen produces limited molecular and functional effects in human cortical organoids.
    Article Snippet: .. The following primary antibodies were used: PAX6 (mouse, 1:250, Abcam ab78545), NeuN (rabbit, 1:200, Cell Signaling, 24307S), MAP2 (chicken, 1:3000, Abcam, ab5392), Ki67 (rabbit, 1:500, Abcam, ab15580), Nestin (mouse, 1:200, Abcam, ab18102), ZO1 (mouse, 1:500, BD Biosciences, BDB610967), and Cleaved Caspase-3 (rabbit, 1: 400, Cell Signalling, #9661). ..

    Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway
    Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), Phospho-p38 MAPK (4511, CST), Phospho-p44/42 MAPK (Erk1/2, 4370, CST), and β-Actin antibody (4967, CST). .. After incubated with appropriate secondary antibodies, the bands were visualized using Clarity Western ECL Substrate (Bio-Rad) and imaged using a chemiluminescence detection system with auto-exposure setting.

    Incubation:

    Article Title: miR-20a-5p inhibition attenuates post-myocardial infarction ventricular remodeling in association with mTORC1/S6 signaling
    Article Snippet: Protein samples (20–40 μg) were separated by SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes (IPVH00010, Millipore, USA). .. Membranes were incubated with antibodies against Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-73364-3 p-S6 (1:2,000, 80130-2-RR, Proteintech), LC3B (1:6,000, 14600-1-AP, Proteintech), p62 (1:10,000, 84826-1- RR, Proteintech), cleaved caspase-3 (1:1,000, #9661, CST), Bcl-2 (1:500, 26593-1-AP, Proteintech), α-SMA (1:1,000, 19245 S, CST), Gal-3 (1:1,000, #89572, CST), and GAPDH (1:5,000, 10494-1-AP, Proteintech), followed by an HRP-conjugated secondary antibody (1:3,000, RGAR001, Proteintech). .. Signals were developed using ECL reagent (34577, Thermo Fisher Scientific, USA) and quantified using Image Lab software.

    Article Title: Radiation-induced AXL upregulation in CCR2 + monocytes promotes cardiac fibrosis.
    Article Snippet: .. Sections were incubated at 4◦C overnight with primary antibodies against α-SMA (1:300, CST, 19245T), Collagen I (1:250, Abcam, AB270993), CD45 (1:500, Abcam, ab10558), cleaved caspase-3 (1:600, CST, 9664T), CCR2 (1:300, Abcam, AB273050), AXL (1:300, CST, 8661T), RFP (1:800, Rockland, 600-401-379), and GAS6 (1:200, Affinity, DF8659). ..

    Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway
    Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), Phospho-p38 MAPK (4511, CST), Phospho-p44/42 MAPK (Erk1/2, 4370, CST), and β-Actin antibody (4967, CST). .. After incubated with appropriate secondary antibodies, the bands were visualized using Clarity Western ECL Substrate (Bio-Rad) and imaged using a chemiluminescence detection system with auto-exposure setting.

    other:

    Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis
    Article Snippet: Cleaved-Caspase 3 , Cell Signalling Technology , 9662 , 17, 19.

    Nonspecific Binding:

    Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway
    Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), Phospho-p38 MAPK (4511, CST), Phospho-p44/42 MAPK (Erk1/2, 4370, CST), and β-Actin antibody (4967, CST). .. After incubated with appropriate secondary antibodies, the bands were visualized using Clarity Western ECL Substrate (Bio-Rad) and imaged using a chemiluminescence detection system with auto-exposure setting.



    Similar Products

    cleaved caspase 3  (Cell Signaling Technology Inc)
    99
    Cell Signaling Technology Inc cleaved caspase 3
    MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+caspase+3/Caspase-3+Antibody/pmc13675316-5-0-3
    Average 99 stars, based on 1 article reviews
    cleaved caspase 3 - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    anti rabbit cleaved caspase 3 asp175  (Cell Signaling Technology Inc)
    99
    Cell Signaling Technology Inc anti rabbit cleaved caspase 3 asp175
    MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
    Anti Rabbit Cleaved Caspase 3 Asp175, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+caspase+3/Cleaved+Caspase-3+(Asp175)+Antibody/pm42883230-257-96-100
    Average 99 stars, based on 1 article reviews
    anti rabbit cleaved caspase 3 asp175 - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    anti cleaved caspase 3  (Cell Signaling Technology Inc)
    99
    Cell Signaling Technology Inc anti cleaved caspase 3
    MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
    Anti Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+caspase+3/Cleaved+Caspase-3+(Asp175)+Antibody/pm42880285-1624-74-76
    Average 99 stars, based on 1 article reviews
    anti cleaved caspase 3 - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    cleaved caspase 3 asp175  (Cell Signaling Technology Inc)
    99
    Cell Signaling Technology Inc cleaved caspase 3 asp175
    MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
    Cleaved Caspase 3 Asp175, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+caspase+3/Cleaved+Caspase-3+(Asp175)+Rabbit+mAb/pm42880254-251-52-59
    Average 99 stars, based on 1 article reviews
    cleaved caspase 3 asp175 - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    cytochrome c  (Cell Signaling Technology Inc)
    99
    Cell Signaling Technology Inc cytochrome c
    MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
    Cytochrome C, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+caspase+3/Cleaved+Caspase-3+(Asp175)+Antibody/pm42878775-73-38-40
    Average 99 stars, based on 1 article reviews
    cytochrome c - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    pa5 143217 anti cleaved caspase 3 cell signaling technology 9661t anti ve cadherin santa cruz biotechnology sc 9989  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology pa5 143217 anti cleaved caspase 3 cell signaling technology 9661t anti ve cadherin santa cruz biotechnology sc 9989
    MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
    Pa5 143217 Anti Cleaved Caspase 3 Cell Signaling Technology 9661t Anti Ve Cadherin Santa Cruz Biotechnology Sc 9989, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+caspase+3/VE-cadherin+Antibody/pm42873637-140-31-39
    Average 96 stars, based on 1 article reviews
    pa5 143217 anti cleaved caspase 3 cell signaling technology 9661t anti ve cadherin santa cruz biotechnology sc 9989 - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    cleaved caspase 3 immunofluorescence  (Proteintech)
    97
    Proteintech cleaved caspase 3 immunofluorescence
    TMZ+TTF treatment enhances apoptotic responses in glioblastoma cells. (A) Immunofluorescence staining of <t>cleaved</t> <t>caspase‐3</t> (red) and nuclei (blue, DAPI) in U251 and SHG140 cells under different treatment conditions. (B) Quantification of cleaved caspase‐3 fluorescence intensity. (C, E) Representative Annexin V‐FITC/PI flow cytometry plots showing the distribution of viable and apoptotic cell populations at 24 and 48 h in U251 and SHG140 cells, respectively. Lower left: Viable cells; lower right: Early apoptotic cells; upper right: Late apoptotic cells; upper left: PI‐positive non‐apoptotic/dead cells. (D, F) Quantitative analysis of viable‐cell fractions derived from Annexin V‐FITC/PI flow cytometry in U251 and SHG140 cells at 24 and 48 h. For cleaved caspase‐3 quantification, three random 20× fields were averaged within each independent experiment. At least 10,000 events were acquired per flow‐cytometry sample. Data are presented as mean ± SD ( n = 3 independent experiments). Statistical analysis was performed using one‐way ANOVA followed by Tukey's multiple comparisons test within each time point. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Cleaved Caspase 3 Immunofluorescence, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+caspase+3/Caspase+3%2Fp17%2Fp19+Antibody/pmc13651720-64-1-28
    Average 97 stars, based on 1 article reviews
    cleaved caspase 3 immunofluorescence - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion

    Journal: Molecular Biology Reports

    Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

    doi: 10.1007/s11033-026-12913-5

    Figure Lengend Snippet: MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion

    Article Snippet: Cleaved-Caspase 3 , Cell Signalling Technology , 9662 , 17, 19.

    Techniques: In Vitro, In Vivo, Western Blot, Analysis, Control, Transfection, Virus, Negative Control

    FOXO1 and PTEN mediate the regulatory effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-FOXO1 or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. (B) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-PTEN or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion

    Journal: Molecular Biology Reports

    Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

    doi: 10.1007/s11033-026-12913-5

    Figure Lengend Snippet: FOXO1 and PTEN mediate the regulatory effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-FOXO1 or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. (B) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-PTEN or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion

    Article Snippet: Cleaved-Caspase 3 , Cell Signalling Technology , 9662 , 17, 19.

    Techniques: Activation Assay, Western Blot, Analysis, Expressing, Transfection, Negative Control

    Akt and mTOR are involved in mediating the effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with MK2206 or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( B ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with rapamycin or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion

    Journal: Molecular Biology Reports

    Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

    doi: 10.1007/s11033-026-12913-5

    Figure Lengend Snippet: Akt and mTOR are involved in mediating the effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with MK2206 or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( B ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with rapamycin or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion

    Article Snippet: Cleaved-Caspase 3 , Cell Signalling Technology , 9662 , 17, 19.

    Techniques: Activation Assay, Western Blot, Analysis, Expressing, Transfection, Negative Control

    TMZ+TTF treatment enhances apoptotic responses in glioblastoma cells. (A) Immunofluorescence staining of cleaved caspase‐3 (red) and nuclei (blue, DAPI) in U251 and SHG140 cells under different treatment conditions. (B) Quantification of cleaved caspase‐3 fluorescence intensity. (C, E) Representative Annexin V‐FITC/PI flow cytometry plots showing the distribution of viable and apoptotic cell populations at 24 and 48 h in U251 and SHG140 cells, respectively. Lower left: Viable cells; lower right: Early apoptotic cells; upper right: Late apoptotic cells; upper left: PI‐positive non‐apoptotic/dead cells. (D, F) Quantitative analysis of viable‐cell fractions derived from Annexin V‐FITC/PI flow cytometry in U251 and SHG140 cells at 24 and 48 h. For cleaved caspase‐3 quantification, three random 20× fields were averaged within each independent experiment. At least 10,000 events were acquired per flow‐cytometry sample. Data are presented as mean ± SD ( n = 3 independent experiments). Statistical analysis was performed using one‐way ANOVA followed by Tukey's multiple comparisons test within each time point. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Tumor Treating Fields Enhance Temozolomide‐Associated Growth Suppression in Glioblastoma Cells and Patient‐Derived Organoids Through Cell‐Cycle Blockade, Apoptosis, and SMAD ‐Associated Signaling

    doi: 10.1002/cns.71195

    Figure Lengend Snippet: TMZ+TTF treatment enhances apoptotic responses in glioblastoma cells. (A) Immunofluorescence staining of cleaved caspase‐3 (red) and nuclei (blue, DAPI) in U251 and SHG140 cells under different treatment conditions. (B) Quantification of cleaved caspase‐3 fluorescence intensity. (C, E) Representative Annexin V‐FITC/PI flow cytometry plots showing the distribution of viable and apoptotic cell populations at 24 and 48 h in U251 and SHG140 cells, respectively. Lower left: Viable cells; lower right: Early apoptotic cells; upper right: Late apoptotic cells; upper left: PI‐positive non‐apoptotic/dead cells. (D, F) Quantitative analysis of viable‐cell fractions derived from Annexin V‐FITC/PI flow cytometry in U251 and SHG140 cells at 24 and 48 h. For cleaved caspase‐3 quantification, three random 20× fields were averaged within each independent experiment. At least 10,000 events were acquired per flow‐cytometry sample. Data are presented as mean ± SD ( n = 3 independent experiments). Statistical analysis was performed using one‐way ANOVA followed by Tukey's multiple comparisons test within each time point. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: For cleaved caspase‐3 immunofluorescence, cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X‐100, blocked with 5% BSA, and incubated overnight at 4°C with anti‐cleaved caspase‐3 antibody (Proteintech, USA; Cat. No. 25546–1‐AP; 1:200).

    Techniques: Immunofluorescence, Staining, Fluorescence, Flow Cytometry, Analysis, Derivative Assay

    ALK5/TGFβRI inhibition partially attenuates TTF‐associated SMAD phosphorylation, growth suppression, and cleaved caspase‐3 induction. (A) Western blot analysis of phosphorylated SMAD2 (p‐SMAD2), phosphorylated SMAD3 (p‐SMAD3), and total SMAD2 in U251 and SHG140 cells treated with vehicle control, TTF, SB525334, or TTF+SB525334. (B) Quantification of p‐SMAD2, p‐SMAD3, and total SMAD2 protein expression levels. Densitometric values for p‐SMAD2, p‐SMAD3, and SMAD2 were normalized to GAPDH. (C, D) CCK‐8 assays measuring cell viability in U251 and SHG140 cells treated with vehicle control, TTF, SB525334, or TTF+SB525334 under the same 72 h assay conditions used in Figure . SB525334 was used at 10 μM as a selective ALK5/TGFβRI inhibitor. (E) Immunofluorescence staining of cleaved caspase‐3 (red) and nuclei (blue, DAPI) in U251 and SHG140 cells under the indicated treatment conditions; merged images are shown. (F) Quantification of cleaved caspase‐3 fluorescence intensity. For cleaved caspase‐3 quantification, three random 20× fields were averaged within each independent experiment. Data are presented as mean ± SD ( n = 3 independent experiments). Statistical analysis was performed using one‐way ANOVA followed by Tukey's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Tumor Treating Fields Enhance Temozolomide‐Associated Growth Suppression in Glioblastoma Cells and Patient‐Derived Organoids Through Cell‐Cycle Blockade, Apoptosis, and SMAD ‐Associated Signaling

    doi: 10.1002/cns.71195

    Figure Lengend Snippet: ALK5/TGFβRI inhibition partially attenuates TTF‐associated SMAD phosphorylation, growth suppression, and cleaved caspase‐3 induction. (A) Western blot analysis of phosphorylated SMAD2 (p‐SMAD2), phosphorylated SMAD3 (p‐SMAD3), and total SMAD2 in U251 and SHG140 cells treated with vehicle control, TTF, SB525334, or TTF+SB525334. (B) Quantification of p‐SMAD2, p‐SMAD3, and total SMAD2 protein expression levels. Densitometric values for p‐SMAD2, p‐SMAD3, and SMAD2 were normalized to GAPDH. (C, D) CCK‐8 assays measuring cell viability in U251 and SHG140 cells treated with vehicle control, TTF, SB525334, or TTF+SB525334 under the same 72 h assay conditions used in Figure . SB525334 was used at 10 μM as a selective ALK5/TGFβRI inhibitor. (E) Immunofluorescence staining of cleaved caspase‐3 (red) and nuclei (blue, DAPI) in U251 and SHG140 cells under the indicated treatment conditions; merged images are shown. (F) Quantification of cleaved caspase‐3 fluorescence intensity. For cleaved caspase‐3 quantification, three random 20× fields were averaged within each independent experiment. Data are presented as mean ± SD ( n = 3 independent experiments). Statistical analysis was performed using one‐way ANOVA followed by Tukey's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: For cleaved caspase‐3 immunofluorescence, cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X‐100, blocked with 5% BSA, and incubated overnight at 4°C with anti‐cleaved caspase‐3 antibody (Proteintech, USA; Cat. No. 25546–1‐AP; 1:200).

    Techniques: Inhibition, Phospho-proteomics, Western Blot, Analysis, Control, Expressing, CCK-8 Assay, Immunofluorescence, Staining, Fluorescence