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cleaved caspase 3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 3
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 17842 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+caspase+3/Cleaved+Caspase-3+(Asp175)+Antibody/10__1038_slash_s41598___026___73232___0-52-25-32
    Average 99 stars, based on 17842 article reviews
    cleaved caspase 3 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: FADS1-mediated Efferocytosis in Tumor-Associated Macrophages Shapes an Immunosuppressive Microenvironment in Gastric Cancer
    Article Snippet: .. After blocking with 3% BSA, sections were incubated overnight at 4°C with primary antibodies against FADS1 (Atlas, HPA042705), cleaved caspase-3 (CST, #9661), IFN-γ (huabio, ET1703-17), and Granzyme B (CST, #46890). ..

    Article Title: The neutrophil-NETs-elastase axis drives pulmonary lymphatic endothelial injury and aggravates acute lung injury.
    Article Snippet: Acute lung injury (ALI) is a severe lung disease marked by strong neutrophil-related inflammation and pulmonary edema.. However, how activated neutrophils damage pulmonary lymphatic function during ALI remains unclear.. In this study, we used an LPS-induced ALI mouse model, pulmonary lymphatic tracing, neutrophil depletion, NET degradation, NE inhibition, LEC experiments, RNA sequencing, and molecular analyses to examine this question.

    Incubation:

    Article Title: FADS1-mediated Efferocytosis in Tumor-Associated Macrophages Shapes an Immunosuppressive Microenvironment in Gastric Cancer
    Article Snippet: .. After blocking with 3% BSA, sections were incubated overnight at 4°C with primary antibodies against FADS1 (Atlas, HPA042705), cleaved caspase-3 (CST, #9661), IFN-γ (huabio, ET1703-17), and Granzyme B (CST, #46890). ..

    Article Title: Species-specific cleavage of the autophagy adaptor p62 dictates responses to TNF.
    Article Snippet: The FFPE sections were dewaxed (Dewax solution, Leica, Cat# AR9222), underwent epitope retrieval (ER2 solution, Leica, Cat# AR9640) for 20 min at 100◦C, rinsed with Leica wash buffer (Leica, Cat# AR9590) before peroxidase blocking using an Intense R kit (Leica, Cat# DS9263). .. Sections were next rinsed with wash buffer before incubation in either cleaved caspase-3 (1/500 dilution, Cell Signaling, Cat# 9661; RRID:AB_2341188) or cleaved caspase-8 (1/200 dilution, Cell Signaling, Cat# 8592S; RRID:AB_10999739) antibody for 30 min. .. Sections were again rinsed with wash buffer and subsequently incubated in rabbit envision secondary antibody (Agilent, Cat# K4003; RRID:AB_2630375) for a further 30 min. After rinsing with wash buffer, liquid DAB (Agilent, Cat# K3468) was applied to the sections for 10 min before washing in water and counterstaining with haematoxylin z (CellPath, Cat# RBA-4201-00A).

    Article Title: The neutrophil-NETs-elastase axis drives pulmonary lymphatic endothelial injury and aggravates acute lung injury.
    Article Snippet: Acute lung injury (ALI) is a severe lung disease marked by strong neutrophil-related inflammation and pulmonary edema.. However, how activated neutrophils damage pulmonary lymphatic function during ALI remains unclear.. In this study, we used an LPS-induced ALI mouse model, pulmonary lymphatic tracing, neutrophil depletion, NET degradation, NE inhibition, LEC experiments, RNA sequencing, and molecular analyses to examine this question.

    Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway
    Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), Phospho-p38 MAPK (4511, CST), Phospho-p44/42 MAPK (Erk1/2, 4370, CST), and β-Actin antibody (4967, CST). .. After incubated with appropriate secondary antibodies, the bands were visualized using Clarity Western ECL Substrate (Bio-Rad) and imaged using a chemiluminescence detection system with auto-exposure setting.

    other:

    Article Title: Protective effect of Zymosan-A against radiation-induced premature ovarian insufficiency in a murine model
    Article Snippet: Cleaved Caspase-3 , Cell Signaling Technology , Cat# 9661; RRID: AB_2341188.

    Nonspecific Binding:

    Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway
    Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), Phospho-p38 MAPK (4511, CST), Phospho-p44/42 MAPK (Erk1/2, 4370, CST), and β-Actin antibody (4967, CST). .. After incubated with appropriate secondary antibodies, the bands were visualized using Clarity Western ECL Substrate (Bio-Rad) and imaged using a chemiluminescence detection system with auto-exposure setting.

    Cell-Signaling:

    Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway
    Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), Phospho-p38 MAPK (4511, CST), Phospho-p44/42 MAPK (Erk1/2, 4370, CST), and β-Actin antibody (4967, CST). .. After incubated with appropriate secondary antibodies, the bands were visualized using Clarity Western ECL Substrate (Bio-Rad) and imaged using a chemiluminescence detection system with auto-exposure setting.



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    Image Search Results


    TMZ+TTF treatment enhances apoptotic responses in glioblastoma cells. (A) Immunofluorescence staining of cleaved caspase‐3 (red) and nuclei (blue, DAPI) in U251 and SHG140 cells under different treatment conditions. (B) Quantification of cleaved caspase‐3 fluorescence intensity. (C, E) Representative Annexin V‐FITC/PI flow cytometry plots showing the distribution of viable and apoptotic cell populations at 24 and 48 h in U251 and SHG140 cells, respectively. Lower left: Viable cells; lower right: Early apoptotic cells; upper right: Late apoptotic cells; upper left: PI‐positive non‐apoptotic/dead cells. (D, F) Quantitative analysis of viable‐cell fractions derived from Annexin V‐FITC/PI flow cytometry in U251 and SHG140 cells at 24 and 48 h. For cleaved caspase‐3 quantification, three random 20× fields were averaged within each independent experiment. At least 10,000 events were acquired per flow‐cytometry sample. Data are presented as mean ± SD ( n = 3 independent experiments). Statistical analysis was performed using one‐way ANOVA followed by Tukey's multiple comparisons test within each time point. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Tumor Treating Fields Enhance Temozolomide‐Associated Growth Suppression in Glioblastoma Cells and Patient‐Derived Organoids Through Cell‐Cycle Blockade, Apoptosis, and SMAD ‐Associated Signaling

    doi: 10.1002/cns.71195

    Figure Lengend Snippet: TMZ+TTF treatment enhances apoptotic responses in glioblastoma cells. (A) Immunofluorescence staining of cleaved caspase‐3 (red) and nuclei (blue, DAPI) in U251 and SHG140 cells under different treatment conditions. (B) Quantification of cleaved caspase‐3 fluorescence intensity. (C, E) Representative Annexin V‐FITC/PI flow cytometry plots showing the distribution of viable and apoptotic cell populations at 24 and 48 h in U251 and SHG140 cells, respectively. Lower left: Viable cells; lower right: Early apoptotic cells; upper right: Late apoptotic cells; upper left: PI‐positive non‐apoptotic/dead cells. (D, F) Quantitative analysis of viable‐cell fractions derived from Annexin V‐FITC/PI flow cytometry in U251 and SHG140 cells at 24 and 48 h. For cleaved caspase‐3 quantification, three random 20× fields were averaged within each independent experiment. At least 10,000 events were acquired per flow‐cytometry sample. Data are presented as mean ± SD ( n = 3 independent experiments). Statistical analysis was performed using one‐way ANOVA followed by Tukey's multiple comparisons test within each time point. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: For cleaved caspase‐3 immunofluorescence, cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X‐100, blocked with 5% BSA, and incubated overnight at 4°C with anti‐cleaved caspase‐3 antibody (Proteintech, USA; Cat. No. 25546–1‐AP; 1:200).

    Techniques: Immunofluorescence, Staining, Fluorescence, Flow Cytometry, Analysis, Derivative Assay

    ALK5/TGFβRI inhibition partially attenuates TTF‐associated SMAD phosphorylation, growth suppression, and cleaved caspase‐3 induction. (A) Western blot analysis of phosphorylated SMAD2 (p‐SMAD2), phosphorylated SMAD3 (p‐SMAD3), and total SMAD2 in U251 and SHG140 cells treated with vehicle control, TTF, SB525334, or TTF+SB525334. (B) Quantification of p‐SMAD2, p‐SMAD3, and total SMAD2 protein expression levels. Densitometric values for p‐SMAD2, p‐SMAD3, and SMAD2 were normalized to GAPDH. (C, D) CCK‐8 assays measuring cell viability in U251 and SHG140 cells treated with vehicle control, TTF, SB525334, or TTF+SB525334 under the same 72 h assay conditions used in Figure . SB525334 was used at 10 μM as a selective ALK5/TGFβRI inhibitor. (E) Immunofluorescence staining of cleaved caspase‐3 (red) and nuclei (blue, DAPI) in U251 and SHG140 cells under the indicated treatment conditions; merged images are shown. (F) Quantification of cleaved caspase‐3 fluorescence intensity. For cleaved caspase‐3 quantification, three random 20× fields were averaged within each independent experiment. Data are presented as mean ± SD ( n = 3 independent experiments). Statistical analysis was performed using one‐way ANOVA followed by Tukey's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Tumor Treating Fields Enhance Temozolomide‐Associated Growth Suppression in Glioblastoma Cells and Patient‐Derived Organoids Through Cell‐Cycle Blockade, Apoptosis, and SMAD ‐Associated Signaling

    doi: 10.1002/cns.71195

    Figure Lengend Snippet: ALK5/TGFβRI inhibition partially attenuates TTF‐associated SMAD phosphorylation, growth suppression, and cleaved caspase‐3 induction. (A) Western blot analysis of phosphorylated SMAD2 (p‐SMAD2), phosphorylated SMAD3 (p‐SMAD3), and total SMAD2 in U251 and SHG140 cells treated with vehicle control, TTF, SB525334, or TTF+SB525334. (B) Quantification of p‐SMAD2, p‐SMAD3, and total SMAD2 protein expression levels. Densitometric values for p‐SMAD2, p‐SMAD3, and SMAD2 were normalized to GAPDH. (C, D) CCK‐8 assays measuring cell viability in U251 and SHG140 cells treated with vehicle control, TTF, SB525334, or TTF+SB525334 under the same 72 h assay conditions used in Figure . SB525334 was used at 10 μM as a selective ALK5/TGFβRI inhibitor. (E) Immunofluorescence staining of cleaved caspase‐3 (red) and nuclei (blue, DAPI) in U251 and SHG140 cells under the indicated treatment conditions; merged images are shown. (F) Quantification of cleaved caspase‐3 fluorescence intensity. For cleaved caspase‐3 quantification, three random 20× fields were averaged within each independent experiment. Data are presented as mean ± SD ( n = 3 independent experiments). Statistical analysis was performed using one‐way ANOVA followed by Tukey's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: For cleaved caspase‐3 immunofluorescence, cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X‐100, blocked with 5% BSA, and incubated overnight at 4°C with anti‐cleaved caspase‐3 antibody (Proteintech, USA; Cat. No. 25546–1‐AP; 1:200).

    Techniques: Inhibition, Phospho-proteomics, Western Blot, Analysis, Control, Expressing, CCK-8 Assay, Immunofluorescence, Staining, Fluorescence